Composite

Part:BBa_K4256177:Design

Designed by: Jingke Wang   Group: iGEM22_UCAS-China   (2022-09-29)


FNR expression system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 651
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 651
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.


Source

It comes from the combination of composite parts BBa_I712074, BBa_K3716001, BBa_K4256133 and BBa_B0012, and the source of each part can be checked.

References